單克隆抗體開發
IMD 在使用雜交瘤技術生成高度特異性 mAb 方面擁有數十年的經驗，成功開發了大量用於研究、臨床診斷和治療應用的 mAb 就證明了這一點。我們知道如何生成對免疫原具有特異性並適用於預期應用的 mAb。我們的服務包括（1）肽抗原的設計和生產； (2) BALB/c小鼠免疫及細胞融合； (3) ELISA或Biacore系統篩選陽性克隆； (4) 亞克隆和擴增； (5) 抗體同種型和表徵； (6)雜交瘤細胞的冷凍保存； (7) 單克隆抗體的批量生產和發酵純化。
我們擁有最先進的 mAb 生產和表徵設施。
Our Services
1
Design and Production of peptide antigens
Design and production of peptide antigens with BSA and KLH conjugation. Gene synthesis, cloning with codon optimization, and recombinant protein expression and purification form selected host cells (E.coli, yeast, insect or mammalian systems). Alternatively, customers can provide their own antigen for immunization and screening.
Approximate Duration: 4-6 weeks
2
Immunization & Titer Evaluation
immunization if BALB/c mice using our optimized in-house protocols. Typically requires 3-4 immunizations to achieve desired serum titers, with additional boost immunizations available if needed. Serum antibody titers are rigorously evaluated by ELISA to selected the best candidates for fusion.
Approximate Duration: 8-10 weeks
3
Fusion, Cloning & Hybridoma Expansion
Splenocytes from mice with the highest serum titers are fused with SP2/0 myeloma cells. Hybridoma supernatants are screened by ELISA to identify positive clones. 2–4 rounds of subcloning ensure clonality and stability. Customers may choose 2 hybridoma clones for further expansion based on titer data, and aliquots of supernatant can be provided for in-house testing.
Approximate Duration: 6 weeks
4
Isotyping, Characterization & Production
Antibody isotyping, affinity, and binding kinetics determined by ELISA and Biacore® systems (GE Biacore 8K). Production options include serum-free medium or mouse ascites fluid (1–2 mg). Purification using Protein-A/G beads or antigen-specific affinity columns. Bulk production and purification with fermentation capabilities (Sartorius Biostat B).
Approximate Duration: 8 weeks
5
Cryopreservation & Long-Term Storage
Cryopreservation of hybridoma cells to ensure long-term viability and security. Stable cell line maintenance for future production needs, providing you with reliable access to your mAb clones for ongoing research and development.