人β2微球蛋白
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介紹
Beta 2 微球蛋白是一種 11 kDa 的蛋白質，與包括淋巴細胞在內的許多細胞的外膜相關。 β2-微球蛋白少量存在於正常人的血清、腦脊液和尿液中，在腎小管蛋白性貧血、腎功能衰竭或腎移植患者的尿液和血漿中含量更高。人 β 2 微球蛋白水平會升高，因為其合成速率增加或腎臟濾過受損。此外，據報導，在類風濕性關節炎和自身免疫性疾病中發現其他血清水平升高。
INTRODUCTION TO THE MOLECULE
Beta 2 microglobulin is an 11 kDa protein associated with the outer membrane of many cells including lymphocytes. Beta 2-microglobulin is present in small amounts in serum, cerebrospinal fluid, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular protein anemia, renal failure, or kidney transplants. Human Beta 2 microglobulin levels can rise either because its rate of synthesis has increased or because of impaired renal filtration. In addition, it has been reported that other increased serum levels are found in rheumatoid arthritis and autoimmune diseases.
DATASHEET
AMINO ACID SEQUENCE
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSENLYFQIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
APPLICATIONS
Standard ELISA test, Western Blot.
FORMULATION
Lyophilized at 1 mg/mL in 50mM phosphate, 10% glycerol, pH 8.0.
RECONSTITUTION
Add deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
STORAGE
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing /thawing cycles.
QUALITY CONTROL TEST
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.