HbA1c is the term known as glycated hemoglobin. Throughout the whole life cycle of red blood cell, HbA1c is formed continuously by the adduction of glucose to the N-terminal of the hemoglobin beta chain. Formation of the sugar-Hb linkage indicates the presence of excessive sugar in the bloodstream, often indicative of diabetes. Therefore, measurement of %HbA1c can also be used to determine three-month average blood sugar level since the average lifespan of a red blood cell is four months. HbA1c level in diabetic subjects will be elevated by 2-3 fold over the levels found in normal individuals. The cut-off value for %HbA1c is 6.5%. The higher the %HbA1c, the higher chance the diabetic subjects are going to develop complications.

 

HbA1c PETIA kit developed by IMD can accurately measure %HbA1c in human whole blood sample.

This assay is a turbidimetric immunoassay for the quantitative measurement of %HbA1c in human whole blood.  A standard or sample is added into a cuvette and mixed with the reaction buffer R1. Total hemoglobin and HbA1c have the same unspecific absorption rate to latex particles. After a short incubation, the test reagent R2 is added into the cuvette and mixed. Latex-HbA1c-mouse anti-human HbA1c antibody complex is formed. Agglutination is formed when goat anti-mouse IgG polyclonal antibody interacts with the monoclonal antibody. The extent to which the microparticles aggregate is quantified by the amount of light scattering measured as absorbance by a chemistry analyzer. The %HbA1c in unknown samples can be interpolated from a reference curve using the standards provided.

Assay procedures may vary depending on the automated chemistry analyzer to be used. A general example of assay procedures is stated as follow:

• Dispense 150µl of R1 into a clean cuvette
• Add 4µl of sample and incubate at 37˚C for 5 minutes
• Further add 50µl of R2
• Read change of absorbance at 660 nm for 5 - 8 minutes after the addition of R2
• Calculate the %HbA1c in unknown sample by interpolation from a reference curve using the standards provided

 

Caution: Different lot of R1, R2, standard and control should not be mixed for use

 

Cat. No.	Size	Hemolysis reagent	Approximately tests
51A1c-05	R1: 15ml, R2: 5ml	50ml	100
51A1c -10	R1: 30ml, R2: 10ml	100ml	200
51A1c -20	R1: 60ml, R2: 20ml	200ml	400
51A1c -50	R1: 150ml, R2: 50ml	500ml	1000
51A1c -100	R1: 300ml, R2: 100ml	1000ml	2000

 

Product	Catalog Number	Format	Method
Calibrator	51Ac1-S1	4 x 0.5 ml
Control	51Ac1-C1	2 x 0.5 ml