高靈敏小鼠胰島素ELISA試劑盒
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化驗 範圍： 0.2 - 7 納克/毫升
樣本量： 10 升
套件尺寸： 96 孔/試劑盒
介紹
胰島素是一種僅在胰腺 β 細胞中產生的肽激素。它由A鍊和B鏈組成，通過兩個二硫鍵連接。
胰島素是負責葡萄糖代謝的主要激素。胰島素分泌受損和胰島素抵抗是 2 型糖尿病 (T2D) 的關鍵原因。測定原理
該測定是雙位點ELISA。微孔板預先塗有抗胰島素的單克隆抗體。將標準品和样品加入孔中，並與與辣根過氧化物酶 (HRP) 酶偶聯的單克隆抗體共同孵育。在去除任何未結合物質的洗滌步驟之後，加入 TMB 底物，顏色與最初結合的胰島素量成比例。停止測定並使用微孔板讀數器測定孔的光密度。由於吸光度的增加與捕獲的胰島素量成正比，因此可以從每個測定中包含的參考曲線中插入未知樣品濃度。
檢測性能
A. 標準曲線的典型代表
以下標準曲線僅供演示。應為每個測定生成標準曲線。
胰島素 (ng/mL)
吸光度
(450 納米)
消隱吸光度
0
0.052
0
0.2
0.063
0.011
0.5
0.098
0.046
1.0
0.197
0.145
2.0
0.558
0.506
3.5
1.23
1.178
7
2.731
2.679
B. 敏感性
該測定法可測量的最低胰島素水平為 0.2 納克/毫升。
C. 精度
測定內精密度（測定內的精密度）CV <6.1%。
測定間精度（測定之間的精度）CV <2.9%。
D. 恢復
通過向樣品中加入不同量的胰島素來確定測定的回收率。將測定中加標樣品的測量濃度與預期濃度進行比較。平均回收率為 91%。
E. 特異性
交叉反應百分比
人胰島素：100%
大鼠胰島素：100%
PUBLICATIONS CITING THIS PRODUCT
- Jiang X, Zhou Y, Wu KK, Chen Z, Xu A, Cheng KK. APPL1 prevents pancreatic beta cell death and inflammation by dampening NFκB activation in a mouse model of type 1 diabetes. Diabetologia. 2017 Mar 1;60(3):464-74.
- Geng L, Liao B, Jin L, Huang Z, Triggle CR, Ding H, Zhang J, Huang Y, Lin Z, Xu A. Exercise alleviates obesity-induced metabolic dysfunction via enhancing FGF21 sensitivity in adipose tissues. Cell reports. 2019 Mar 5;26(10):2738-52.
- Huang Q, Gong Q, Wen T, Feng S, Xu J, Liu J, Han X, Liu Q, Hu J, Zhu L. Loss of LAMTOR1 in pancreatic β‑cells increases glucose‑stimulated insulin secretion in mice. Int J Mol Med. 2020 Jan;45(1):23-32. doi: 10.3892/ijmm.2019.4409. Epub 2019 Nov 21.
- Yang BC, Wu SY, Leung PS. Alcohol ingestion induces pancreatic islet dysfunction and apoptosis via mediation of FGF21 resistance. Annals of translational medicine. 2020 Mar;8(6).
- Sun H, Wei G, Liu H, Xiao D, Huang J, Lu J, Miao J, Liu J, Chen S. Inhibition of XBP1s ubiquitination enhances its protein stability and improves glucose homeostasis. Metabolism. 2020 Apr 1;105:154046.
- Breznik JA, Foley KP, Maddiboina D, Schertzer JD, Sloboda DM, Bowdish DM. Effects of Obesity-Associated Chronic Inflammation on Peripheral Blood Immunophenotype Are Not Mediated by TNF in Female C57BL/6J Mice. ImmunoHorizons. 2021 Jun 4;5(6):370-83.
- Wang D, Mai Q, Yang X, Chi X, Li R, Jiang J, Luo L, Fang X, Yun P, Liang L, Yang G, Song K, Fang L, Chen Y, Zhang Y, He Y, Li N, Pan Y. Microduplication of 16p11.2 locus potentiates hypertrophic obesity in association with imbalanced triglyceride metabolism in white adipose tissue. Molecular Nutrition & Food Research. 2022 Jan 24, 66, 2100241.
- Geng L, Liao B, Jin L, Yu J, Zhao X, Zhao Y, Zhong L, Wang B, Li J, Liu J, Yang JK, Jia W, Lian Q, Xu A. β-Klotho promotes glycolysis and glucose-stimulated insulin secretion via GP130. Nature Metabolism.2022 May 12, 4, 608–626
- Zhong J, Cheung CYY, Su X, Lee CH, Ru Y, Fong CHY, Liu Y, Cheung CKY, Lam KSL, Cai Z, Xu A. Specific triacylglycerol, diacylglycerol, and lyso-phosphatidylcholine species for the prediction of type 2 diabetes: a ~ 16-year prospective study in Chinese. Cardiovasc Diabetol. 2022 Nov 5;21(1):234.
- Kei N, Cheung KK, Ma KL, Yau TK, Lauw S, Wong VWS, You L, Cheung PCK. Effects of Oat β-Glucan and Inulin on Alleviation of Nonalcoholic Steatohepatitis Aggravated by Circadian Disruption in C57BL/6J Mice. Journal of Agricultural and Food Chemistry. 2024 Feb 21;72(7):3520-3535.
Tips of choosing our Insulin products
Insulin ELISA Kit
Assay Range (ng/mL)
Sample Volume Required Per Well (uL)
Assay Length (h)
Ultra sensitive (Rat Cat.: 33380/Mouse Cat.: 32380)
0.025-1.5
25
2.5
High sensitive (Rat Cat.:33270/Mouse Cat.:32270)
0.2-7
10
2
High sensitive Plus(Mouse only Cat.: 32393)
0.2-7
5
2.5
Wide range (Rat Cat.: 33100/Mouse Cat.:32100)
1-50
5
2