脂聯素是一種專門在脂肪組織中表達的脂肪因子，具有有效的抗糖尿病、抗動脈粥樣硬化和抗炎功能。
在人類中，血清脂聯素水平降低與體重指數 (BMI) 增加、胰島素敏感性降低、血脂狀況不佳、炎症增加以及發生 2 型糖尿病、高血壓和冠心病的風險增加有關

該測定是使用針對人脂聯素的單克隆抗體的定量夾心ELISA。免疫板預先塗有對人脂聯素特異的單克隆抗體。標準品和样品被移入孔中，任何存在的人脂聯素都被固定化抗體結合。洗去任何未結合的物質後，將辣根過氧化物酶 (HRP) 連接的特異於人脂聯素的單克隆抗體加入孔中。在最後的洗滌步驟之後，加入 HRP 底物溶液，顏色與最初結合的人脂聯素的量成比例。 停止測定並使用酶標儀測定孔的光密度。由於吸光度的增加與捕獲的人脂聯素的量成正比，因此可以從每個測定中包含的參考曲線中插入未知樣品濃度。

A. 標準曲線的典型代表

以下標準曲線僅供演示。應為每組樣品測定生成標準曲線。 

B. 靈敏度

該測定法可檢測到的人脂聯素的最低水平為 1.56 ng/mL。

C. 特異性

在該測定中使用的抗體對特定於人脂聯素和不與小鼠和大鼠脂聯素和其它細胞因子或激素分子交叉反應包括人抵抗素，TNF一個，ANGPTL4，胰島素，瘦素和IL6。

D. 精度

該 ELISA 試劑盒的測定變化在四個具有不同濃度內源性脂聯素的人血清樣品上進行了研究。變異內的平均值是根據指定樣品的每個測定中五次重複測定的結果計算的。每個樣品的變化之間的平均值是根據四個單獨測定的結果計算的，每個測定中都有重複的樣品。

E. 恢復

將不同量的人脂聯素添加到三個人血清樣品中，並在三個獨立的測定中測定脂聯素含量。回收百分比 = 觀察到的脂聯素濃度/預期的脂聯素濃度 x 100%。

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