Expressed in E.coli with total 271 AA. Mw: 30.4 KDa (calculated).
N-terminal 6xHis-tag, EK and TEV cleavage site, 44 extra AA (highlighted). Recombinant antigen for research use or manufacturing only.

FGF-23 is a bone-derived hormone that acts in the kidney to regulate phosphate homeostasis and vitamin D metabolism. The signaling receptor for FGF-23, ɑ Klotho-FGFR1 (IIIc) complex, is an essential regulator of the renal sodium phosphate co-transporter and key vitamin D-metabolizing enzymes CYP27B1 and CYP24A1. Mature human FGF-23 contains an atypical (very low affinity) heparin binding site (aa 134-162), a proteolytic cleavage site (Arg179-Ser180), and multiple O-linked glycosylation sites with Thr178 being of particular importance. O-linked glycosylation at Thr178 blocks the cleavage of FGF-23, thereby preventing loss of FGF-23 activity. This recombinant human FGF23 bears mutation at 179th aa from arginine to glutamine preventing proteolytic cleavage.

Lyophilized at 1 mg/mL in NaCl 300mM, KCl 2.7mM, Na2HPO4 10mM, KH2PO4 1.8mM, pH 7.4.

Cell culture, animal studies, ELISA and Western blotting.

BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.

Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C.
Avoid repeated freezing/thawing cycles.