胰島素是一種僅在胰腺 β 細胞中產生的肽激素。它由A鍊和B鏈組成，通過兩個二硫鍵連接。
胰島素是負責葡萄糖代謝的主要激素。胰島素分泌受損和胰島素抵抗是 2 型糖尿病 (T2D) 的關鍵原因。

該測定是雙位點ELISA。微孔板預先塗有抗胰島素的單克隆抗體。將標準品和样品加入孔中，並與與辣根過氧化物酶 (HRP) 酶偶聯的單克隆抗體共同孵育。在去除任何未結合物質的洗滌步驟之後，加入 TMB 底物，顏色與最初結合的胰島素量成比例。停止測定並使用微孔板讀數器測定孔的光密度。由於吸光度的增加與捕獲的胰島素量成正比，因此可以從每個測定中包含的參考曲線中插入未知樣品濃度。

A. 標準曲線的典型代表

以下標準曲線僅供演示。應為每個測定生成標準曲線。

B. 敏感性

該測定法可測量的最低胰島素水平為 3 µU/mL。

C. 精度

測定內精密度（測定內的精密度）CV < 3.4%。

測定間精密度（測定之間的精密度）CV <5.0%。

D. 恢復

通過向樣品中加入不同量的胰島素來確定測定的回收率。將測定中加標樣品的測量濃度與預期濃度進行比較。平均回收率為 92%。

E. 特異性

交叉反應百分比

小鼠胰島素：100%

大鼠胰島素：100%

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