Human Interleukin 2 (hIL-2)
Type: | Recombinant | Cat. No.: | 41B050 |
Source: | E. coli | Size: | 0.1 mg |
Species: | Human | Purity: | >95% |
Description
Recombinant Human Interleukin 2 without Signal peptide (AA21-153), total 134 amino acids (AA). N-terminal His-tag removed. Mw: 15.4 kDa (calculated). 1 extra AA left (In bold).
Introduction to the Molecule
Interleukin 2 (IL-2) is an immuno-modulatory cytokine which exerts its biological activity primarily on T cells promoting proliferation and maturation. It has been found to stimulate growth and differentiation of B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Amino Acid Sequence
AAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
Formulation
Lyophilized in 1 mg/mL in PBS.
Endotoxin Level
<0.2 EU/ug.
Applications
Cell culture, animal studies, ELISA and Western blotting.
Reconstitution
Add sterile deionized water to prepare a working stock solution of approximately 1 mg/mL and let the lyophilized pellet dissolve completely.
Storage
Store lyophilized protein at –20°C. Aliquot reconstituted protein and store at –80°C. Avoid repeated freezing/thawing cycles
Biological Activity Test
The biological activity of Human IL-2 is determined by proliferation assay using Jurkat T cells.