脂聯素是一種專門在脂肪組織中表達的脂肪因子，具有有效的抗糖尿病、抗動脈粥樣硬化和抗炎功能。
在人類中，血清脂聯素水平降低與體重指數 (BMI) 增加、胰島素敏感性降低、血脂狀況不佳、炎症增加以及發生 2 型糖尿病、高血壓和冠心病的風險增加有關

該測定是一種夾心 ELISA，使用針對小鼠脂聯素的親和純化多克隆抗體。免疫板預塗有抗小鼠脂聯素捕獲抗體。標準品和样品被移入孔中，任何存在的小鼠脂聯素都被固定化抗體結合。洗去任何未結合的物質後，將辣根過氧化物酶 (HRP) 連接的小鼠脂聯素特異性多克隆抗體加入孔中。在最後的洗滌步驟之後，加入 HRP 底物溶液，顏色與最初結合的小鼠脂聯素的量成比例。停止測定並使用酶標儀測定孔的光密度。由於吸光度的增加與捕獲的小鼠脂聯素的量成正比，因此可以從每個測定中包含的參考曲線中插入未知樣品濃度。

一種。 標準曲線的典型代表

以下標準曲線僅供演示。應為每組樣品測定生成標準曲線。

B. 敏感性

該測定法可檢測到的最低水平的小鼠脂聯素為 3.12 ng/mL。

C. 特異性

該檢測中使用的抗體對小鼠脂聯素具有特異性，不會與人脂聯素和其他細胞因子或激素分子發生交叉反應。

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