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小鼠 FGF-21 ELISA 試劑盒

小鼠 FGF-21 ELISA 試劑盒

庫存單位: 32180

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化驗 範圍:  31.2 - 2000pg/mL

套件尺寸:  96 孔/試劑盒

其他名稱:  UNQ3115/PRO10196

  • 介紹

    FGF21 是一種主要在肝臟中產生的代謝激素。它通過多種組織中的多效作用調節葡萄糖和脂質代謝,並在各種壓力下防止代謝損傷。
     在人類中,高循環 FGF21 水平存在於肥胖和多種心臟代謝疾病中, 包括代謝綜合徵、2型糖尿病、非酒精性脂肪肝和冠狀動脈疾病。血清 FGF21 是這些疾病早期檢測和風險預測的潛在生物標誌物。

  • 測定原理

    該測定是定量夾心ELISA。免疫板預塗有小鼠 FGF-21 特異性兔多克隆抗體。標準品和样品被吸入孔中,任何存在的小鼠 FGF-21 都被固定的抗體結合。洗去任何未結合的物質後,將生物素標記的小鼠 FGF-21 特異性多克隆抗體加入孔中。在去除任何未結合試劑的洗滌步驟後,加入鏈黴親和素-HRP 偶聯物 (STP-HRP)。在最後一個洗滌步驟後,加入 HRP 底物溶液,顏色與最初結合的小鼠 FGF-21 的量成比例。停止測定並使用酶標儀測定孔的光密度。由於吸光度的增加與捕獲的小鼠 FGF-21 的量成正比,因此可以從每個測定中包含的參考曲線中插入未知樣品濃度。

  • 檢測性能

    一種。 標準曲線的典型代表

    以下標準曲線僅供演示。應為每組樣品測定生成標準曲線。 

    FGF-21 (pg/mL)

    吸光度 (450 nm)

    消隱吸光度

    0

    0.109

    0

    31.2

    0.132

    0.023

    62.5

    0.158

    0.049

    125

    0.209

    0.1

    250

    0.309

    0.2

    500

    0.571

    0.462

    1000

    1.031

    0.992

    2000年

    1.9

    1.791

     

    B. 敏感性

    該測定法可檢測到的小鼠 FGF-21 的最低水平為 31.2 皮克/毫升。

     

    C. 特異性

    重組蛋白的交叉反應

    分析物交叉反應
    人 FGF-21
    鼠標 Mup-1
    鼠標 LCN2
    小鼠脂聯素
    鼠標 ANGPL4

     

    D. 精度

    測定內精密度(測定內的精密度)CV <8%。

    測定間精密度(測定之間的精度)CV <10%。

     

    E. 恢復

    通過向樣品中加入不同量的 FGF21 來確定測定的回收率。將測定中加標樣品的測量濃度與預期濃度進行比較。平均回收率為 92%。

     

    F. 線性

    為了評估分析的線性,將具有高水平 FGF-21 的樣品用 1x 分析緩衝液連續稀釋,以產生值在分析動態範圍內的樣品。

    稀釋測量 (pg/mL)預期 (pg/mL)恢復 (%)
    1/2 1360 1360 100
    1/4 680 680 101
    1/8 380 340 111
    1/16 200 170 115
  • PUBLICATIONS CITING THIS PRODUCT

    1. Li H, Gao Z, Zhang J, Ye X, Xu A, Ye J, Jia W. Sodium butyrate stimulates expression of fibroblast growth factor 21 in liver by inhibition of histone deacetylase 3. Diabetes. 2012 Apr 1;61(4):797-806.
    2. Lin Z, Tian H, Lam KS, Lin S, Hoo RC, Konishi M, Itoh N, Wang Y, Bornstein SR, Xu A, Li X. Adiponectin mediates the metabolic effects of FGF21 on glucose homeostasis and insulin sensitivity in mice. Cell metabolism. 2013 May 7;17(5):779-89.
    3. Huang Z, Zhong L, Lee JT, Zhang J, Wu D, Geng L, Wang Y, Wong CM, Xu A. The FGF21-CCL11 axis mediates beiging of white adipose tissues by coupling sympathetic nervous system to type 2 immunity. Cell metabolism. 2017 Sep 5;26(3):493-508.
    4. Li H, Wu G, Fang Q, Zhang M, Hui X, Sheng B, Wu L, Bao Y, Li P, Xu A, Jia W. Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat. Nature communications. 2018 Jan 18;9(1):1-6.
    5. Pan X, Shao Y, Wu F, Wang Y, Xiong R, Zheng J, Tian H, Wang B, Wang Y, Zhang Y, Han Z. Fgf21 prevents angiotensin ii-induced hypertension and vascular dysfunction by activation of ace2/angiotensin-(1–7) axis in mice. Cell metabolism. 2018 Jun 5;27(6):1323-37.
    6. Pan Y, Wang B, Zheng J, Xiong R, Fan Z, Ye Y, Zhang S, Li Q, Gong F, Wu C, Lin Z. Pancreatic fibroblast growth factor 21 protects against type 2 diabetes in mice by promoting insulin expression and secretion in a PI3K/Akt signaling‐dependent manner. Journal of cellular and molecular medicine. 2019 Feb;23(2):1059-71.
    7. Pan Y, Hui X, Hoo RL, Ye D, Chan CY, Feng T, Wang Y, Lam KS, Xu A. Adipocyte-secreted exosomal microRNA-34a inhibits M2 macrophage polarization to promote obesity-induced adipose inflammation. The Journal of clinical investigation. 2019 Feb 1;129(2):834-49.
    8. Geng L, Liao B, Jin L, Huang Z, Triggle CR, Ding H, Zhang J, Huang Y, Lin Z, Xu A. Exercise alleviates obesity-induced metabolic dysfunction via enhancing FGF21 sensitivity in adipose tissues. Cell reports. 2019 Mar 5;26(10):2738-52.
    9. Yang BC, Wu SY, Leung PS. Alcohol ingestion induces pancreatic islet dysfunction and apoptosis via mediation of FGF21 resistance. Annals of translational medicine. 2020 Mar;8(6).
    10. Zhang Y, Cao X, Chen L, Qin Y, Xu Y, Tian Y, Chen L. Exposure of female mice to perfluorooctanoic acid suppresses hypothalamic kisspeptin‐reproductive endocrine system through enhanced hepatic fibroblast growth factor 21 synthesis, leading to ovulation failure and prolonged dioestrus. Journal of neuroendocrinology. 2020 May;32(5):e12848.
    11. Zhou Y, Li C, Wang X, Deng P, He W, Zheng H, Zhao L, Gao H. Integration of FGF21 Signaling and Metabolomics in High-Fat Diet-Induced Obesity. Journal of Proteome Research. 2021 Jul 9;20(8):3900-12.
    12. Liu D, Gu J, Shao W, Pang J, Qian X, Jin T. Comparison of metabolic beneficial effects of Liraglutide and Semaglutide in male C57BL/6J mice. Canadian Journal of Diabetes. 2021 Sep 8.
    13. Wang Y, Zhang Y, Shi Z, Di T, Yu W, Chen L. Exposure of male mice to perfluorooctanoic acid induces anxiety-like behaviors by increasing corticotropin-releasing factor in the basolateral amygdala complex. Chemosphere. 2022 Jan 1;287:132170
    14. Badakhshi Y, Shao W, Liu D, Tian L, Pang J, Gu J, Hu J, Jin T. Estrogen-Wnt signaling cascade regulates expression of hepatic fibroblast growth factor 21. American Journal of Physiology-Endocrinology and Metabolism. 2021 Aug 03; 321:2, E292-E304.
    15. Qiu H, Song E, Hu Y, et al. Hepatocyte-Secreted Autotaxin Exacerbates Nonalcoholic Fatty Liver Disease Through Autocrine Inhibition of the PPARα/FGF21 Axis. Cellular and Molecular Gastroenterology and Hepatology. 2022 Aug:S2352-345X(22)00170-9
    16. Yu J, Laybuttab DR, Youngson NA, Morris MJ. Concurrent betaine administration enhances exercise-induced improvements to glucose handling in obese mice. Nutrition, Metabolism and Cardiovascular Diseases. 2022 Aug 18.
    17. Badakhshi Y, Shao W, Liu D, Tian L, Pang J, Gu J, Hu J, Jin T. Estrogen-Wnt signaling cascade regulates expression of hepatic fibroblast growth factor 2 Am J Physiol Endocrinol Metab. 2021 Aug 1;321(2):E292-E304.
    18. Long X, Liu D, Gao Q, Ni J, Qian L, Ni Y, Fang Q, Jia W, Li H. Bifidobacterium adolescentis Alleviates Liver Steatosis and Steatohepatitis by Increasing Fibroblast Growth Factor 21 Sensitivity. Front Endocrinol (Lausanne). 2021 Dec 30;12:773340.
    19. Ni Y, Qian L, Siliceo SL, Long X, Nychas E, Liu Y, Ismaiah MJ, Leung H, Zhang L, Gao Q, Wu Q, Zhang Y, Jia X, Liu S, Yuan R, Zhou L, Wang X, Li Q, Zhao Y, El-Nezami H, Xu A, Xu G, Li H, Panagiotou G, Jia W. Resistant starch decreases intrahepatic triglycerides in patients with NAFLD via gut microbiome alterations. Cell Metab. 2023 Sep 5;35(9):1530-1547.e8.
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