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Mouse IL6-Ra ELISA Kit

Mouse IL6-Ra ELISA Kit

庫存單位: 32B090

 Download Datasheet PDF ★

 

 

Assay range: 46.9 - 3000pg/mL

Kit Size: 96 wells/kit

 

  • INTRODUCTION

    IL-6 receptor alpha (IL-6Ra) is a transmembrane subunit of the receptor for Interleukin-6. IL-6 is crucial for the development of the acute phase response, inflammation, hematopoiesis, bone metabolism, and cancer 1. Acute inflammation is triggered by IL-6, and it then progresses to either acquired immunity or chronic inflammation 2-3. It contributes to inflammatory bowel disease, obesity, insulin resistance, arthritis, sepsis, and atherosclerosis when it is dysregulated 4-5.

  • PRINCIPLE OF THE ASSAY

    This assay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The microtiter plate is pre-coated with a polyclonal antibody specific for mouse IL-6Ra. Standards and samples are pipetted into the wells and any mouse IL-6Ra present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse IL-6Ra is added to the wells. After wash step to remove any unbound reagents, streptavidin-horseradish peroxidase conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of mouse IL-6Ra bound initially. The assay is stopped, and the optical density of the wells is determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse IL-6Ra, the unknown sample concentration can be interpolated from a reference curve included in each assay.

  • ASSAY PERFORMANCE

    TYPICAL STANDARD CURVE

    The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.

    Mouse IL-6Ra

    (pg/mL)

    Absorbance

    (450 nm)

    Blanked Absorbance

    0

    0.128

    0

    46.9

    0.182

    0.054

    93.8

    0.232

    0.104

    187.5

    0.347

    0.219

    375

    0.54

    0.412

    750

    0.89

    0.762

    1500

    1.569

    1.441

    3000

    2.801

    2.673

    Mouse IL-6Ra standard curve (4-parameter)

     

     

    ASSAY CHARACTERISTICS

     

    A. Sensitivity

    The lowest level of mouse IL-6Ra that can be detected by this assay is 46.9 pg/mL.

     

    B. Precision

    Intra-assay Precision (Precision within an assay) C.V. <3.4%. Inter-assay Precision (Precision between assays) C.V. <6.8%.

     

  • INTENDED USE

    This Mouse IL-6Ra ELISA kit is designed for quantification of mouse IL-6Ra in serum, plasma and cell culture supernate samples.

$420.00價格

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