PLA2G7, also known as lipoprotein‑associated phospholipase A2 (Lp‑PLA2), is a secreted, calcium‑independent enzyme involved in phospholipid metabolism and inflammatory regulation. It hydrolyzes the sn‑2 ester bond of phospholipids and inactivates platelet‑activating factor (PAF), a potent mediator of inflammation. Beyond PAF degradation, PLA2G7 also targets oxidatively modified phospholipids within LDL and HDL particles, helping prevent the accumulation of pro‑inflammatory lipids during oxidative stress. Genetic defects in PLA2G7 can lead to platelet‑activating factor acetylhydrolase deficiency, contributing to heightened inflammatory responses and related clinical conditions.

This assay is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA). The microtiter plate is pre-coated with a polyclonal antibody specific for mouse PLA2G7. Standards and samples are pipetted into the wells and any mouse TFF2 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin labelled polyclonal antibody specific for mouse PLA2G7 is added to the wells. After wash step to remove any unbound reagents, streptavidin-horseradish peroxidase conjugate (STP-HRP) is added. After the last wash step, an HRP substrate solution is added and color develops in proportion to the amount of mouse PLA2G7 bound initially. The assay is stopped, and the optical density of the wells is determined using a microplate reader. Since the increases in absorbance are directly proportional to the amount of captured mouse PLA2G7, the unknown sample concentration can be interpolated from a reference curve included in each assay.

TYPICAL STANDARD CURVE

The following standard curve is provided for demonstration only. A standard curve should be generated for each set of sample assay.