Stanniocalcin 2 (STC2) is a glycosylated, disulfide-linked, homodimeric hormone initially identified in the corpus of stannous--a small endocrine organ of bony fish . In mammals, STC2 is widely expressed in different tissues as well as in tumour cells. STC2 has been implicated in diverse biological processes, including calcium and phosphate regulation, cytoprotection, cell development, and angiogenesis , and is a potential prognostic marker for a variety of cancers . STC2 overexpression in MC3T3 cells facilitated osteoblast differentiation and mineralization through regulation of ERK1/2 phosphorylation, suggesting its involvement in bone metabolism . Additionally, STC2 has been shown to attenuate ovarian progesterone biosynthesis via the PKA pathway, accompanied by the inhibition of follicle-stimulating hormone (FSH)-induced Cyp1a1 and 3β-hydroxysteroid dehydrogenase expression. Furthermore, STC2 is a potential metabolic hormone involved in the regulation of glucose homeostasis. In both brown adipose tissue and liver of mice, STC2 expression is induced by fasting, and treatment with recombinant STC2 increases glycogen levels in the fasting state and exerts opposite effects on gluconeogenesis in rat hepatocytes in the fasting and fed states.

Antigen affinity purification

Recombinant mouse STC2 protein expressed in mammalian cells.

The antibody detects mouse STC2 in western blot and Elisa.

Liquid in phosphate-buffered saline (PBS). Store at –20°C for less than one week. For long-term storage, aliquot and freeze at -70°C. Avoid repeated freeze/defrost cycles.

Western blot – This antibody can be used as a primary antibody in western blot to detect mouse STC2.
ELISA - This antibody can be used as a capture antibody in mouse STC2 ELISA.