Rheumatoid Factor (RF) is an abnormal antibody made by human immune system which can bind to other healthy antibodies and destroy the function of patients’ normal immune system. High levels of rheumatoid factor in the plasma are most often related to autoimmune diseases, such as rheumatoid arthritis and Sjogren's syndrome. Therefore, RF is a more accurate and sensitive biomarker for diagnosing autoimmune diseases. In clinical research, the increase in serum secretion of RF has not be only indicated rheumatoid arthritis and Sjogren's syndrome, but also associated with a number of other diseases, e.g. Cancer, Chronic infections, Inflammatory lung diseases, Mixed connective tissue disease, Systemic lupus erythematosus. Thus, monitoring RF levels has a great clinic significance for general health screening, especially in older age group. The reference value of the RF in plasma is < 20 IU/ml.

該測定是用於定量測量人血清和血漿中的類風濕因子的免疫比濁法。將標準品或樣品加入比色皿並與反應緩衝液 R1 混合。短暫孵育後，將測試試劑R2，即包被有正常人IgG抗體的微粒懸液，其中Fc部分可以被類風濕因子高度特異性識別，加入比色杯中並混合。標準品或樣品中類風濕因子的存在會導致免疫顆粒聚集。微粒聚集的程度通過由化學分析儀測量為吸光度的光散射量來量化。未知樣品中類風濕因子的濃度可以使用提供的標準從參考曲線內插。

TYPICAL STANDARD KITS

檢測程序可能因所使用的自動化學分析儀而異。分析程序的一般示例如下所述：

1. 將 150µl R1 分裝到干淨的比色皿中

2. 加入 3µl 血清或血漿樣本或類風濕因子校準品，37°C 孵育 5 分鐘

3. 再加入 50µl R2

4. 加入 R2 後 8 分鐘讀取 570 nm 處的吸光度變化

5. 使用提供的標準，通過參考曲線的插值計算未知樣品中類風濕因子的濃度

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