TNF-α, Human
Species: | Human |
Expression System: | E.coli |
MW: | 17 kDa (reducing) |
Accession: | P01375 |
Cat No. | UA040018 |
Endotoxin: | ＜1 EU/μg |
Purity: | >95% as determined by SDS-PAGE |
INTRODUCTION
Tumor Necrosis Factor alpha (TNF-α), is an inflammatory cytokine produced by macrophages/monocytes during acute inflammation and is responsible for a diverse range of signaling events within cells, leading to necrosis or apoptosis. Active TNF-alpha and other members of the TNF superfamily exist as a homotrimer with high structural homology. TNFα activates signals through two receptors, TNFR1, which is expressed on most cell types, and TNF-R2, which is expressed mainly on immune cells.
Amino Acid Sequence
Val77-Leu233
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIALPhysical Appearance:
Lyophilized powder
Buffer:
PBS, pH 7.4
Reconstitution:
Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
- 1 week, 2 to 8 °C under sterile conditions after reconstitution.
Note: Please avoid repeated freeze-thaw cycles