胰島素是一種僅在胰腺 β 細胞中產生的肽激素。它由A鍊和B鏈組成，通過兩個二硫鍵連接。
胰島素是負責葡萄糖代謝的主要激素。胰島素分泌受損和胰島素抵抗是 2 型糖尿病 (T2D) 的關鍵原因。

該測定是雙位點ELISA。微孔板預先塗有抗胰島素的單克隆抗體。將標準品和样品加入孔中，並與與辣根過氧化物酶 (HRP) 酶偶聯的單克隆抗體共同孵育。在去除任何未結合物質的洗滌步驟之後，加入 TMB 底物，顏色與最初結合的胰島素量成比例。停止測定並使用微孔板讀數器測定孔的光密度。由於吸光度的增加與捕獲的胰島素量成正比，因此可以從每個測定中包含的參考曲線中插入未知樣品濃度。

A.標準曲線的典型表示

以下標準曲線僅供演示。應為每個測定生成標準曲線。

B.敏感性

該測定可測量的最低胰島素水平為 0.025 納克/毫升。

C. 精度

測定內精密度（測定內的精密度）CV < 2.9%。

測定間精密度（測定之間的精密度）CV <4.9%。

D. 恢復

通過向樣品中加入不同量的胰島素來確定測定的回收率。將測定中加標樣品的測量濃度與預期濃度進行比較。平均回收率為 92%。

E. 特異性

交叉反應百分比

人胰島素：100%

大鼠胰島素：100%

1. Shu L, Hoo RL, Wu X, Pan Y, Lee IP, Cheong LY, Bornstein SR, Rong X, Guo J, Xu A. A-FABP mediates adaptive thermogenesis by promoting intracellular activation of thyroid hormones in brown adipocytes. Nature communications. 2017 Jan 27;8(1):1-6.
2. Fan W, Zhang R, Han D, Jiang Z, Li S, Zhang J, Li Y, Wang Y, Cao F. Reduced Sirtuin1 signalling exacerbates diabetic mice hindlimb ischaemia injury and inhibits the protective effect of a liver X receptor agonist. Journal of cellular and molecular medicine. 2020 May;24(10):5476-90.
3. Wang B, Lin H, Li X, Lu W, Kim JB, Xu A, Cheng KK. The adaptor protein APPL2 controls glucose-stimulated insulin secretion via F-actin remodeling in pancreatic β-cells. Proceedings of the National Academy of Sciences. 2020 Nov 10;117(45):28307-15.
4. Zhang J, Ni Y, Qian L, Fang Q, Zheng T, Zhang M, Gao Q, Zhang Y, Ni J, Hou X, Bao Y. Decreased Abundance of Akkermansia muciniphila Leads to the Impairment of Insulin Secretion and Glucose Homeostasis in Lean Type 2 Diabetes. Advanced Science. 2021 Jun 4:2100536.
5. So WY, Liu WN, Teo AK, Rutter GA, Han W. Paired box 6 programs essential exocytotic genes in the regulation of glucose-stimulated insulin secretion and glucose homeostasis. Science Translational Medicine. 2021 Jun 30;13(600):eabb1038.
6. Liu D, Gu J, Shao W, Pang J, Qian X, Jin T. Comparison of metabolic beneficial effects of Liraglutide and Semaglutide in male C57BL/6J mice. Canadian Journal of Diabetes. 2021 Sep 8.
7. Xiao Y, Shu L, Wu X, Liu Y, Cheong LY, Liao B, Xiao X, Hoo RLC, Zhou Z, Xu A. Fatty acid binding protein 4 promotes autoimmune diabetes by recruitment and activation of pancreatic islet macrophages. JCI Insight. 2021 Mar 9, 6(7):e141814.
8. Wong THT, Wan JMF, Tse IMY, Sit WH, Louie JCY. Consumption of decaffeinated coffee with milk and sugar added before a high-glycemic-index meal lowers postprandial glucose surge when compared with consuming it after the meal. Human Nutrition & Metabolism. 2021 Jun, 200124.
9. Wu KKL, Long K, Lin H, Siu PMF, Hoo RLC, Ye D, Xu A, Cheng KKY. The APPL1-Rab5 axis restricts NLRP3 inflammasome activation through early endosomal-dependent mitophagy in macrophages. Nat Commun. 2021 Nov 17;12(1):6637.
10. Bai N, Lu X, Jin L, Alimujiang M, Ma J, Hu F, Xu Y, Sun J, Xu J, Zhang R, Han J, Hu C, Yang Y. CLSTN3 gene variant associates with obesity risk and contributes to dysfunction in white adipose tissue. Mol Metab. 2022 Sep;63:101531.
11. Ni Y, Qian L, Siliceo SL, Long X, Nychas E, Liu Y, Ismaiah MJ, Leung H, Zhang L, Gao Q, Wu Q, Zhang Y, Jia X, Liu S, Yuan R, Zhou L, Wang X, Li Q, Zhao Y, El-Nezami H, Xu A, Xu G, Li H, Panagiotou G, Jia W. Resistant starch decreases intrahepatic triglycerides in patients with NAFLD via gut microbiome alterations. Cell Metabolism. 2023 Sep 5;35(9):1530-1547.e8.